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Description: |
xi, 388 pages : illustrations ; 26 cm |
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Bibliography Note: |
Includes bibliographical references and index. |
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Contents Note: |
Contents: 1. The basics of molecular biology -- 2. The tools of the molecular biologist -- 3. General preparations, procedures, and considerations for use of manual -- 4. Cloning vectors and bacterial cells -- 5. Preparation for DNA from eukaryotic cells -- 6. Probing nucleic acids with labeled synthetic probes -- 7. Probing nucleic acids with plasmid-derived probes -- 8. Plasmid DNA preparation -- 9. DNA restriction fragment preparation -- 10. Purification of DNA -- 11. Preparation and analysis of RNA from eukaryotic cells -- 12. Preparation of DNA from bacteriophage clones -- 13. Cloning DNA from the eukaryotic genome -- 14. cDNA cloning into [lambda]gt10 and [lambda]gt11 -- 15. Subcloning into plasmids -- 16. M13 cloning and sequencing -- 17. Further characterization of cloned DNA -- 18. Transfection of mammalian cells in culture -- 19. Protein methods -- 20. General methods -- 21. Specialized methods. |
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Additional Physical Forms: |
Online version: Davis, Leonard G. Basic methods in molecular biology. New York : Elsevier, ©1986 (OCoLC)571304664 |