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Description: |
3 volumes : illustrations ; 23 cm |
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Bibliography Note: |
Includes bibliographical references and indexes. |
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Contents Note: |
Contents: Volume I. 1. The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries / K. Kaiser and N.E. Murray -- 2. Constructing and screening cDNA libraries in [lambda]gt10 and [lambda]gt11 / T.V. Huynh, R.A. Young and R.W. Davis -- 3. An alternative procedure for the synthesis of double-stranded cDNA for cloning in phage and plasmid vectors / C.J. Watson and J.F. Jackson -- 4. Immunological detection of chimeric [beta]-galactosidases expressed by plasmid vectors / M. Koenen, H.-W. Griesser and B. Müller-Hill -- 5. The pEMBL family of single-stranded vectors / L. Dente, M. Sollazzo, C. Baldari, G. Cesareni and R. Cortese -- 6. Techniques for transformation of E. coli / D. Hanahan -- 7. The use of genetic markers for the selection and allelic exchange of in vitro induced mutations that do not have a phenotype in E. coli / G. Cesareni, C. Traboni, G. Ciliberto, L. Dente and R. Cortese -- 8. The oligonucleotide-directed construction of mutations in recombinant filamentous phage / H.-J. Fritz -- 9. Broad host range cloning vectors for gram negative bacteria / F.C.H. Franklin -- |
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Volume II. 1. Bacillus cloning methods / K.G. Hardy -- 2. Gene cloning in streptomyces / I.S. Hunter -- 3. Cloning in yeast / R. Rothstein -- 4. Genetic engineering of plants / C. Lichtenstein and J. Draper -- 5. P element mediated germ line transformation of Drosophila / R.E. Karess -- 6. High efficiency gene transfer into mammalian cells / C. Gorman -- 7. The construction and characterisation of vaccinia virus recombinants expressing foreign genes / M. Mackett, G.L. Smith and B. Moss -- 8. Bovine papilloma virus DNA: a eukaryotic cloning vector / M.S. Campo -- |
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Volume III. 1. Application of plasmids containing promoters specific for phage-encoded RNA polymers / Peter F.R. Little and Ian J. Jackson -- 2. Choice and use of cosmid vectors / Peter F.R. Little -- 3. Genetic approaches to the cloning, modification and characterization of cosmid clones and clone libraries / Annemarie Poustka and Hans Lehrach -- 4. The purification of eukaryotic polypeptides expressed in Escherichia coli / Fiona A.O. Marston -- 5. Production and purification of polyclonal antibodies to the foreign segment of [beta]-galactosidase fusion proteins / Sean B. Carroll and Allen Laughon -- 6. Production of monoclonal antibodies against fusion proteins produced in Escherichia coli / Sarah E. Mole and David P. Lane -- 7. Expression and secretion of foreign genes in yeast / Bruce L.A. Carter [and 5 others] -- 8. The use of vectors based on gene amplification for the expression of cloned genes in mammalian cells / Christopher R. Bebbington and Christopher C.G. Hentschel -- 9. Retroviral vectors / Anthony M.C. Brown and Michael R.D. Scott -- 10. The production of transgenic mice by the microinjection of cloned DNA into fertilized one-cell eggs / David Murphy and Jennifer Hanson. |
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Additional Physical Forms: |
Online version: DNA cloning. Oxford ; Washington, DC : IRL Press, ©1985-©1987 (OCoLC)642426988 |
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Elect. Loc./Access: |
Publisher description http://catdir.loc.gov/catdir/enhancements/fy0635/85202319-d.html |
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Name Added Entry: |
Glover, David M., editor. edt |
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Series Added Entry: |
Practical approach series. |