|
Description: |
11 pages : illustrations, tables ; 26 cm. |
|
|
With Note: |
With: Methods for simultaneous determination and identification of MS-222 and metabolites in fish tissues / by Charles W. Luhning. (No. 51). |
|
|
Bibliography Note: |
Includes bibliographical references (pages 10-11). |
|
|
Summary, Etc. Note: |
Abstract: Striped bass anesthetized in a 100-mg/liter solution of MS-222 at 17.5 degrees Centigrade contained an average of 57.9 microgram/gram of MS-222 and 23.3 microgram/gram of m-aminobenzoic acid residues in the muscle tissue immediately after a 30-minute exposure to the drug (0-hour withdrawal samples). After this tissue was homogenized and stored in a refrigerator for 1 week at 1.7 degrees Centigrade, residues were 100 percent m-aminobenzoic acid. Fish anesthetized with benzocaine were treated in a like manner still contained residues of benzocaine and a small amount of p-aminobenzoic acid (3.4 percent) after storage. The ester and acid residues of both anesthetics decreased steadily with length of recovery time. The residues were measured by a modified Bratton-Marshall colorimetric method and confirmed by thin-layer chromatography. |